Molecular Basis of Inward Rectification
نویسندگان
چکیده
منابع مشابه
Molecular Basis of Inward Rectification
Polyamines cause inward rectification of (Kir) K+ channels, but the mechanism is controversial. We employed scanning mutagenesis of Kir6.2, and a structural series of blocking diamines, to combinatorially examine the role of both channel and blocker charges. We find that introduced glutamates at any pore-facing residue in the inner cavity, up to and including the entrance to the selectivity fil...
متن کاملMolecular basis of inward rectification: structural features of the blocker defined by extended polyamine analogs.
Polyamines cause inward rectification of Kir K(+) channels by blocking deep within the channel pore. We investigated structural constraints of polyamine block of strongly rectifying mutant K(ATP) channels (Kir6.2[L164C,N160D,C166S] + SUR1). We studied three groups of polyamine analogs: 1) conformationally restricted linear tetra-amines with a cycloalkyl or alkene group between the second and th...
متن کاملMolecular basis of inward rectification: Polyamine interaction sites located by combined channel and ligand mutagenesis
Polyamines cause inward rectification of (Kir) K channels, but the mechanism is controversial. We employed scanning mutagenesis of Kir6.2, and a structural series of blocking diamines, to combinatorially examine the role of both channel and blocker charges. We find that introduced glutamates at any pore-facing residue in the inner cavity, up to and including the entrance to the selectivity filt...
متن کاملStructural Basis of Inward Rectification Cytoplasmic Pore of the G Protein-Gated Inward Rectifier GIRK1 at 1.8 Å Resolution
Inward rectifier K(+) channels govern the resting membrane voltage in many cells. Regulation of these ion channels via G protein-coupled receptor signaling underlies the control of heart rate and the actions of neurotransmitters in the central nervous system. We have determined the protein structure formed by the intracellular N- and C termini of the G protein-gated inward rectifier K(+) channe...
متن کاملMechanism of Inward Rectification in Kir Channels
Two recent papers published in the Journal of General Physiology (Guo and Lu, 2003; Guo et al., 2003) address the mechanism of inward rectification by polyamines in Kir2.1 channels. In these two papers, Guo et al. (2003) extensively analyze channel block by monoamines, diamines (DAs), and polyamines of varying length and charge, using thermodynamic mutant cycles to calculate interaction energie...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 2004
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.200409159